TIRFM

Movies showing IFT and protein transport in flagella of Chlamydomonas reinhardtii


Intraflagellar transport (IFT)

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IFT20-mCherry expressed in ift20-1 null mutant imaged by TIRFM in real time.


Tubulin transport by IFT

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Simultaneous imaging of mNeonGreen-tagged alpha-tubulin (left) and IFT20-mCherry (right) in a cell with growing cilia.


Tubulin transport in growing cilia

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Cilia were amputated from sfGFP-tubulin expressing cell and the cell was allowed to partially regrow the cilia prior to TIRF analysis. Then, both cilia were bleached and the reentry of unbleached GFP-tubulin from the cell body was imaged. Note tubulin transport by IFT and incorporation of tubulin into the distal parts of the growing cilia.

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