TIRF microscopy of fluorescent beads. The laser spot with a diameter of ~5 µm is displayed in red.
TIRF microscopy of a cell expressing sfGFP-tagged alpha-tubulin. A portion of the flagellum is bleach using a focused laser beam.
We are grateful to Drs. Ahmet Yildiz (Berkeley) and Peter Kner (UGA) for advice on how to install the bleaching laser.